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puuv strains  (DNASTAR)


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    DNASTAR puuv strains
    Puuv Strains, supplied by DNASTAR, used in various techniques. Bioz Stars score: 99/100, based on 5593 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 5593 article reviews
    puuv strains - by Bioz Stars, 2026-06
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    Hantavirus survival in gastric juice. Infectious dose of 10 6 <t>Puumala</t> <t>virus</t> particles was suspended to pure gastric juice set to pH 1–7 with NaOH for 1, 10, or 15 min. After given incubation intervals the gastric juice was neutralized with NaOH and the virus suspension was used to infect VERO-E6 cells for focus titration of infectious particles ( n = 4). As at low pH the inactivation activity was expected to be high pH 2 was omitted to save valuable sample volume.
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    Hantavirus survival in gastric juice. Infectious dose of 10 6 <t>Puumala</t> <t>virus</t> particles was suspended to pure gastric juice set to pH 1–7 with NaOH for 1, 10, or 15 min. After given incubation intervals the gastric juice was neutralized with NaOH and the virus suspension was used to infect VERO-E6 cells for focus titration of infectious particles ( n = 4). As at low pH the inactivation activity was expected to be high pH 2 was omitted to save valuable sample volume.
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    ifa slides with puuv (strain sotkamo)-infected vero e6 cells  (EUROIMMUN)
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    Immunoreactivity of antisera collected from mice immunized either with full-length His-tagged SNV/ANDV N proteins (A) or non-His-tagged chimeric VLPs harbouring a 120-aa-long PUUV N protein segment (B) compared to recombinant N proteins of Sin Nombre virus (SNV), Andes virus (ANDV), Puumala virus strains Kazan (PUUV-Kaz), Sotkamo (PUUV-Sot) and Vranica-Hällnäs (PUUV-Vra), Hantaan virus <t>(HTNV)</t> and Dobrava-Belgrade virus, strain Slovenia (DOBV-Slo). As a negative control, yeast-expressed His-tagged N proteins of rabies virus (RABV) and human parainfluenza virus type 3 (hPIV3) were used
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    Western blot analysis of the cross-reactivity of the mAbs 4H3 (B) and 7G2 (C) with N proteins of Sin Nombre virus (lane 1), Andes virus (lane 2), Puumala virus (PUUV), strain Kazan (lane 3), PUUV, strain Sotkamo (lane 4), PUUV, strain Vranica/Hällnäs (lane 5), Tula virus (lane 6), Hantaan virus (lane 7), Dobrava-Belgrade virus, strain Slovenia (lane 8), and <t>Seoul</t> <t>virus</t> (lane 9). Lane 10, crude lysate of non-transformed yeast cells. Lane M, PageRulerTM Prestained Protein Ladder (UAB Fermentas, Vilnius, Lithuania, #SM0671). A, as a control, the same yeast cell crude lysates were run in a SDS polyacrylamide gel and stained with Coomassie blue
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    sequences from puuv strain umea/hu, puuv strain kazan, m-segment, l-segment  (Eurofins)
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    Western blot analysis of the cross-reactivity of the mAbs 4H3 (B) and 7G2 (C) with N proteins of Sin Nombre virus (lane 1), Andes virus (lane 2), Puumala virus (PUUV), strain Kazan (lane 3), PUUV, strain Sotkamo (lane 4), PUUV, strain Vranica/Hällnäs (lane 5), Tula virus (lane 6), Hantaan virus (lane 7), Dobrava-Belgrade virus, strain Slovenia (lane 8), and <t>Seoul</t> <t>virus</t> (lane 9). Lane 10, crude lysate of non-transformed yeast cells. Lane M, PageRulerTM Prestained Protein Ladder (UAB Fermentas, Vilnius, Lithuania, #SM0671). A, as a control, the same yeast cell crude lysates were run in a SDS polyacrylamide gel and stained with Coomassie blue
    Sequences From Puuv Strain Umea/Hu, Puuv Strain Kazan, M Segment, L Segment, supplied by Eurofins, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    vero e6 cell line adapted puuv strain kazan  (ATCC)
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    Cells grown on coverslips were infected at a MOI of 0.1, fixed and stained (in green) for <t>PUUV</t> <t>Kazan-E6</t> at 5 days post infection (dpi) (A), or for PUUV Umeå at 11 dpi (B). Nuclei were visualized with DAPI staining (blue). Cultivation media from VEFs infected with PUUV Kazan-E6 (C) or PUUV Umeå (D) were collected at the indicated time points post infection and the virus titre, determined as focus forming units (FFU)/ml, were assayed. Graphs represent the means from one of two or more different experiments.
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    ATCC puumala virus puuv sotkamo strain
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    Hantavirus survival in gastric juice. Infectious dose of 10 6 Puumala virus particles was suspended to pure gastric juice set to pH 1–7 with NaOH for 1, 10, or 15 min. After given incubation intervals the gastric juice was neutralized with NaOH and the virus suspension was used to infect VERO-E6 cells for focus titration of infectious particles ( n = 4). As at low pH the inactivation activity was expected to be high pH 2 was omitted to save valuable sample volume.

    Journal: Frontiers in Microbiology

    Article Title: Gastrointestinal Tract As Entry Route for Hantavirus Infection

    doi: 10.3389/fmicb.2017.01721

    Figure Lengend Snippet: Hantavirus survival in gastric juice. Infectious dose of 10 6 Puumala virus particles was suspended to pure gastric juice set to pH 1–7 with NaOH for 1, 10, or 15 min. After given incubation intervals the gastric juice was neutralized with NaOH and the virus suspension was used to infect VERO-E6 cells for focus titration of infectious particles ( n = 4). As at low pH the inactivation activity was expected to be high pH 2 was omitted to save valuable sample volume.

    Article Snippet: For in vitro studies: PUUV strain Sotkamo was grown on Vero-E6 cells (ATCC CRL-1586; American Type Culture Collection, Manassas, USA) under standard cell culture conditions.

    Techniques: Virus, Incubation, Suspension, Titration, Activity Assay

    (A) Growth kinetics of hantaviruses in Caco-2 cells. Caco-2 cells growing on cell culture slides were infected with Puumala virus at MOI of 0.1. Viral replication was monitored by qPCR in culture medium and cells ( n = 3). (B) Translocation of hantavirus through polarized Caco-2 monolayers. Caco-2 cells growing on filter inserts for at least 3 weeks were infected by Puumala virus at MOI 0.1. Apical and basal medium were collected at 24, 120, and 240 h p.i. and investigated for viral replication by qPCR ( n = 3).

    Journal: Frontiers in Microbiology

    Article Title: Gastrointestinal Tract As Entry Route for Hantavirus Infection

    doi: 10.3389/fmicb.2017.01721

    Figure Lengend Snippet: (A) Growth kinetics of hantaviruses in Caco-2 cells. Caco-2 cells growing on cell culture slides were infected with Puumala virus at MOI of 0.1. Viral replication was monitored by qPCR in culture medium and cells ( n = 3). (B) Translocation of hantavirus through polarized Caco-2 monolayers. Caco-2 cells growing on filter inserts for at least 3 weeks were infected by Puumala virus at MOI 0.1. Apical and basal medium were collected at 24, 120, and 240 h p.i. and investigated for viral replication by qPCR ( n = 3).

    Article Snippet: For in vitro studies: PUUV strain Sotkamo was grown on Vero-E6 cells (ATCC CRL-1586; American Type Culture Collection, Manassas, USA) under standard cell culture conditions.

    Techniques: Cell Culture, Infection, Virus, Translocation Assay

    Intracellular localization of hantavirus in Caco-2 cells. Confocal laser-scanning microscopy of infected cell monolayers. Caco-2 cells growing on coverslips were infected with Puumala virus at MOI of 0.1. The cells were fixed with methanol-free formaldehyde and stained with antibodies against PUUV nucleocapsid protein (red) and early endosomal antigen 1 (EEA1, green). Cell nuclei were stained by DAPI (blue).

    Journal: Frontiers in Microbiology

    Article Title: Gastrointestinal Tract As Entry Route for Hantavirus Infection

    doi: 10.3389/fmicb.2017.01721

    Figure Lengend Snippet: Intracellular localization of hantavirus in Caco-2 cells. Confocal laser-scanning microscopy of infected cell monolayers. Caco-2 cells growing on coverslips were infected with Puumala virus at MOI of 0.1. The cells were fixed with methanol-free formaldehyde and stained with antibodies against PUUV nucleocapsid protein (red) and early endosomal antigen 1 (EEA1, green). Cell nuclei were stained by DAPI (blue).

    Article Snippet: For in vitro studies: PUUV strain Sotkamo was grown on Vero-E6 cells (ATCC CRL-1586; American Type Culture Collection, Manassas, USA) under standard cell culture conditions.

    Techniques: Confocal Laser Scanning Microscopy, Infection, Virus, Staining

    Epithelial barrier dysfunction in infected Caco-2 monolayers. Caco-2 cells growing on filter inserts were infected by Puumala virus at MOI 0.1. Transepithelial electrical resistance (TER) was measured during infection with chopstick electrodes ( n = 4).

    Journal: Frontiers in Microbiology

    Article Title: Gastrointestinal Tract As Entry Route for Hantavirus Infection

    doi: 10.3389/fmicb.2017.01721

    Figure Lengend Snippet: Epithelial barrier dysfunction in infected Caco-2 monolayers. Caco-2 cells growing on filter inserts were infected by Puumala virus at MOI 0.1. Transepithelial electrical resistance (TER) was measured during infection with chopstick electrodes ( n = 4).

    Article Snippet: For in vitro studies: PUUV strain Sotkamo was grown on Vero-E6 cells (ATCC CRL-1586; American Type Culture Collection, Manassas, USA) under standard cell culture conditions.

    Techniques: Infection, Virus

    (A) Tight junction disturbance. Caco-2 cells grown on filter inserts were infected with Puumala virus at MOI of 0.1 and analyzed by confocal laser-scanning microscopy. At 48 h cells were fixed and stained for PUUV nucleocapsid protein (red) and zonula occludens protein 1 (green). Cell nuclei were stained by DAPI (blue). (B) Cytoskeleton rearrangements in infected Caco-2 monolayers. Caco-2 cells were infected with PUUV at MOI of 0.1. At different time points the cells were fixed with methanol-free formaldehyde and stained with antibodies against PUUV nucleocapsid protein (red) and actin (white). Cell nuclei were stained by DAPI (blue). (C) Leaks in Caco-2 cells monolayers at high MOI. Caco-2 cells grown on coverslips were infected with Puumala virus at MOI of 1.0. At different time points the cells were fixed and stained with antibodies against Puumala virus nucleocapsid protein (red) and zonula occludens protein 1 (green) and analyzed by confocal laser-scanning microscopy. Mock control is shown at 96 h.

    Journal: Frontiers in Microbiology

    Article Title: Gastrointestinal Tract As Entry Route for Hantavirus Infection

    doi: 10.3389/fmicb.2017.01721

    Figure Lengend Snippet: (A) Tight junction disturbance. Caco-2 cells grown on filter inserts were infected with Puumala virus at MOI of 0.1 and analyzed by confocal laser-scanning microscopy. At 48 h cells were fixed and stained for PUUV nucleocapsid protein (red) and zonula occludens protein 1 (green). Cell nuclei were stained by DAPI (blue). (B) Cytoskeleton rearrangements in infected Caco-2 monolayers. Caco-2 cells were infected with PUUV at MOI of 0.1. At different time points the cells were fixed with methanol-free formaldehyde and stained with antibodies against PUUV nucleocapsid protein (red) and actin (white). Cell nuclei were stained by DAPI (blue). (C) Leaks in Caco-2 cells monolayers at high MOI. Caco-2 cells grown on coverslips were infected with Puumala virus at MOI of 1.0. At different time points the cells were fixed and stained with antibodies against Puumala virus nucleocapsid protein (red) and zonula occludens protein 1 (green) and analyzed by confocal laser-scanning microscopy. Mock control is shown at 96 h.

    Article Snippet: For in vitro studies: PUUV strain Sotkamo was grown on Vero-E6 cells (ATCC CRL-1586; American Type Culture Collection, Manassas, USA) under standard cell culture conditions.

    Techniques: Infection, Virus, Confocal Laser Scanning Microscopy, Staining, Control

    (A) Intragastral infection of hamster by Puumala virus. Syrian hamsters (animal identification numbers given on the x-axis) were infected with either 1,000 plaque forming units (PFU), 10,000 PFU or 10,000 PFU γ-irradiated PUUV. Thirty five days post infection hamsters were bled to test for seroconversion by N-ELISA. Dots represent hamsters for which subsequent Andes virus challenge was lethal. Specific OD sum is the sum of the optical densities (ODs) greater than background, and represents the area under the titer curve. (B) Survival curves for hamsters “vaccinated” intragastrically with Puumala virus. Forty two days post intragastric Puumala virus challenge, the same hamsters were challenged with 200 PFU ANDV i.m.

    Journal: Frontiers in Microbiology

    Article Title: Gastrointestinal Tract As Entry Route for Hantavirus Infection

    doi: 10.3389/fmicb.2017.01721

    Figure Lengend Snippet: (A) Intragastral infection of hamster by Puumala virus. Syrian hamsters (animal identification numbers given on the x-axis) were infected with either 1,000 plaque forming units (PFU), 10,000 PFU or 10,000 PFU γ-irradiated PUUV. Thirty five days post infection hamsters were bled to test for seroconversion by N-ELISA. Dots represent hamsters for which subsequent Andes virus challenge was lethal. Specific OD sum is the sum of the optical densities (ODs) greater than background, and represents the area under the titer curve. (B) Survival curves for hamsters “vaccinated” intragastrically with Puumala virus. Forty two days post intragastric Puumala virus challenge, the same hamsters were challenged with 200 PFU ANDV i.m.

    Article Snippet: For in vitro studies: PUUV strain Sotkamo was grown on Vero-E6 cells (ATCC CRL-1586; American Type Culture Collection, Manassas, USA) under standard cell culture conditions.

    Techniques: Infection, Virus, Irradiation, Enzyme-linked Immunosorbent Assay

    Immunoreactivity of antisera collected from mice immunized either with full-length His-tagged SNV/ANDV N proteins (A) or non-His-tagged chimeric VLPs harbouring a 120-aa-long PUUV N protein segment (B) compared to recombinant N proteins of Sin Nombre virus (SNV), Andes virus (ANDV), Puumala virus strains Kazan (PUUV-Kaz), Sotkamo (PUUV-Sot) and Vranica-Hällnäs (PUUV-Vra), Hantaan virus (HTNV) and Dobrava-Belgrade virus, strain Slovenia (DOBV-Slo). As a negative control, yeast-expressed His-tagged N proteins of rabies virus (RABV) and human parainfluenza virus type 3 (hPIV3) were used

    Journal: Archives of virology

    Article Title: Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples

    doi: 10.1007/s00705-010-0879-6

    Figure Lengend Snippet: Immunoreactivity of antisera collected from mice immunized either with full-length His-tagged SNV/ANDV N proteins (A) or non-His-tagged chimeric VLPs harbouring a 120-aa-long PUUV N protein segment (B) compared to recombinant N proteins of Sin Nombre virus (SNV), Andes virus (ANDV), Puumala virus strains Kazan (PUUV-Kaz), Sotkamo (PUUV-Sot) and Vranica-Hällnäs (PUUV-Vra), Hantaan virus (HTNV) and Dobrava-Belgrade virus, strain Slovenia (DOBV-Slo). As a negative control, yeast-expressed His-tagged N proteins of rabies virus (RABV) and human parainfluenza virus type 3 (hPIV3) were used

    Article Snippet: Indirect immunofluorescence assay (IFA) was done using commercial IFA slides with PUUV (strain Hällnäs B1 83–223L)-, HTNV (strain 76–118)- and SEOV (strain R22)-infected Vero E6 cells (Progen Biotechnik, Heidelberg, Germany) and PUUV (strain Sotkamo)-, DOBV (strain Slovenia)-, Saaremaa virus-, SEOV-, HTNV- and SNV-infected Vero E6 cells (EuroImmun, Lübeck, Germany) as recommended by the manufacturers.

    Techniques: Recombinant, Virus, Negative Control

    The reactivity of mAbs with recombinant hantavirus N proteins in western blot and strip immunoblot assays

    Journal: Archives of virology

    Article Title: Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples

    doi: 10.1007/s00705-010-0879-6

    Figure Lengend Snippet: The reactivity of mAbs with recombinant hantavirus N proteins in western blot and strip immunoblot assays

    Article Snippet: Indirect immunofluorescence assay (IFA) was done using commercial IFA slides with PUUV (strain Hällnäs B1 83–223L)-, HTNV (strain 76–118)- and SEOV (strain R22)-infected Vero E6 cells (Progen Biotechnik, Heidelberg, Germany) and PUUV (strain Sotkamo)-, DOBV (strain Slovenia)-, Saaremaa virus-, SEOV-, HTNV- and SNV-infected Vero E6 cells (EuroImmun, Lübeck, Germany) as recommended by the manufacturers.

    Techniques: Recombinant, Western Blot, Stripping Membranes

    The reactivity of mAbs with recombinant yeast-expressed hantavirus N proteins in ELISA

    Journal: Archives of virology

    Article Title: Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples

    doi: 10.1007/s00705-010-0879-6

    Figure Lengend Snippet: The reactivity of mAbs with recombinant yeast-expressed hantavirus N proteins in ELISA

    Article Snippet: Indirect immunofluorescence assay (IFA) was done using commercial IFA slides with PUUV (strain Hällnäs B1 83–223L)-, HTNV (strain 76–118)- and SEOV (strain R22)-infected Vero E6 cells (Progen Biotechnik, Heidelberg, Germany) and PUUV (strain Sotkamo)-, DOBV (strain Slovenia)-, Saaremaa virus-, SEOV-, HTNV- and SNV-infected Vero E6 cells (EuroImmun, Lübeck, Germany) as recommended by the manufacturers.

    Techniques: Recombinant

    Reactivity of N-protein-specific mAbs with hantavirus-infected Vero E6 cells in commercial and in-house indirect IFA

    Journal: Archives of virology

    Article Title: Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples

    doi: 10.1007/s00705-010-0879-6

    Figure Lengend Snippet: Reactivity of N-protein-specific mAbs with hantavirus-infected Vero E6 cells in commercial and in-house indirect IFA

    Article Snippet: Indirect immunofluorescence assay (IFA) was done using commercial IFA slides with PUUV (strain Hällnäs B1 83–223L)-, HTNV (strain 76–118)- and SEOV (strain R22)-infected Vero E6 cells (Progen Biotechnik, Heidelberg, Germany) and PUUV (strain Sotkamo)-, DOBV (strain Slovenia)-, Saaremaa virus-, SEOV-, HTNV- and SNV-infected Vero E6 cells (EuroImmun, Lübeck, Germany) as recommended by the manufacturers.

    Techniques: Virus

    Multiple amino acid (aa) sequence alignment of the amino-terminal region (aa 1–120) of the N proteins of Puumala virus strains Kazan (PUUV-Kaz, GenBank accession No. Z84204), P-360 (PUUVP360, L11347), Sotkamo (PUUV-Sot, X61035), Vranica/Hällnäs (PUUV-Vra, U14137), Tula virus, strain Moravia (TULV, Z69991), Prospect Hill virus (PHV, M34011), Bayou virus (BAYV, L36929), Muleshoe virus (MULEV, U54575), Sin Nombre virus (SNV, NC_005216), Andes virus (ANDV, AF004660), Rio Mamore virus (RMV, U52136), Hantaan virus, strain Fojnica (HTNV, M14626), Seoul virus (SEOV, AY273791) and Dobrava-Belgrade virus, strain Slovenia (DOBV-Slo, L41916). The alignment was generated using ClustalW software. “*” indicates identical aa residues in all sequences in the alignment, “:” indicates conserved substitutions, and “.” indicates semi-conserved substitutions in the alignment

    Journal: Archives of virology

    Article Title: Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples

    doi: 10.1007/s00705-010-0879-6

    Figure Lengend Snippet: Multiple amino acid (aa) sequence alignment of the amino-terminal region (aa 1–120) of the N proteins of Puumala virus strains Kazan (PUUV-Kaz, GenBank accession No. Z84204), P-360 (PUUVP360, L11347), Sotkamo (PUUV-Sot, X61035), Vranica/Hällnäs (PUUV-Vra, U14137), Tula virus, strain Moravia (TULV, Z69991), Prospect Hill virus (PHV, M34011), Bayou virus (BAYV, L36929), Muleshoe virus (MULEV, U54575), Sin Nombre virus (SNV, NC_005216), Andes virus (ANDV, AF004660), Rio Mamore virus (RMV, U52136), Hantaan virus, strain Fojnica (HTNV, M14626), Seoul virus (SEOV, AY273791) and Dobrava-Belgrade virus, strain Slovenia (DOBV-Slo, L41916). The alignment was generated using ClustalW software. “*” indicates identical aa residues in all sequences in the alignment, “:” indicates conserved substitutions, and “.” indicates semi-conserved substitutions in the alignment

    Article Snippet: Indirect immunofluorescence assay (IFA) was done using commercial IFA slides with PUUV (strain Hällnäs B1 83–223L)-, HTNV (strain 76–118)- and SEOV (strain R22)-infected Vero E6 cells (Progen Biotechnik, Heidelberg, Germany) and PUUV (strain Sotkamo)-, DOBV (strain Slovenia)-, Saaremaa virus-, SEOV-, HTNV- and SNV-infected Vero E6 cells (EuroImmun, Lübeck, Germany) as recommended by the manufacturers.

    Techniques: Sequencing, Virus, Generated, Software

    Western blot analysis of the cross-reactivity of the mAbs 4H3 (B) and 7G2 (C) with N proteins of Sin Nombre virus (lane 1), Andes virus (lane 2), Puumala virus (PUUV), strain Kazan (lane 3), PUUV, strain Sotkamo (lane 4), PUUV, strain Vranica/Hällnäs (lane 5), Tula virus (lane 6), Hantaan virus (lane 7), Dobrava-Belgrade virus, strain Slovenia (lane 8), and Seoul virus (lane 9). Lane 10, crude lysate of non-transformed yeast cells. Lane M, PageRulerTM Prestained Protein Ladder (UAB Fermentas, Vilnius, Lithuania, #SM0671). A, as a control, the same yeast cell crude lysates were run in a SDS polyacrylamide gel and stained with Coomassie blue

    Journal: Archives of virology

    Article Title: Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples

    doi: 10.1007/s00705-010-0879-6

    Figure Lengend Snippet: Western blot analysis of the cross-reactivity of the mAbs 4H3 (B) and 7G2 (C) with N proteins of Sin Nombre virus (lane 1), Andes virus (lane 2), Puumala virus (PUUV), strain Kazan (lane 3), PUUV, strain Sotkamo (lane 4), PUUV, strain Vranica/Hällnäs (lane 5), Tula virus (lane 6), Hantaan virus (lane 7), Dobrava-Belgrade virus, strain Slovenia (lane 8), and Seoul virus (lane 9). Lane 10, crude lysate of non-transformed yeast cells. Lane M, PageRulerTM Prestained Protein Ladder (UAB Fermentas, Vilnius, Lithuania, #SM0671). A, as a control, the same yeast cell crude lysates were run in a SDS polyacrylamide gel and stained with Coomassie blue

    Article Snippet: Indirect immunofluorescence assay Indirect immunofluorescence assay (IFA) was done using commercial IFA slides with PUUV (strain Hällnäs B1 83–223L)-, HTNV (strain 76–118)- and SEOV (strain R22)-infected Vero E6 cells (Progen Biotechnik, Heidelberg, Germany) and PUUV (strain Sotkamo)-, DOBV (strain Slovenia)-, Saaremaa virus-, SEOV-, HTNV- and SNV-infected Vero E6 cells (EuroImmun, Lübeck, Germany) as recommended by the manufacturers.

    Techniques: Western Blot, Virus, Transformation Assay, Control, Staining

    The reactivity of mAbs with recombinant hantavirus N proteins in western blot and strip immunoblot assays

    Journal: Archives of virology

    Article Title: Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples

    doi: 10.1007/s00705-010-0879-6

    Figure Lengend Snippet: The reactivity of mAbs with recombinant hantavirus N proteins in western blot and strip immunoblot assays

    Article Snippet: Indirect immunofluorescence assay Indirect immunofluorescence assay (IFA) was done using commercial IFA slides with PUUV (strain Hällnäs B1 83–223L)-, HTNV (strain 76–118)- and SEOV (strain R22)-infected Vero E6 cells (Progen Biotechnik, Heidelberg, Germany) and PUUV (strain Sotkamo)-, DOBV (strain Slovenia)-, Saaremaa virus-, SEOV-, HTNV- and SNV-infected Vero E6 cells (EuroImmun, Lübeck, Germany) as recommended by the manufacturers.

    Techniques: Recombinant, Western Blot, Stripping Membranes

    The reactivity of mAbs with recombinant yeast-expressed hantavirus N proteins in ELISA

    Journal: Archives of virology

    Article Title: Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples

    doi: 10.1007/s00705-010-0879-6

    Figure Lengend Snippet: The reactivity of mAbs with recombinant yeast-expressed hantavirus N proteins in ELISA

    Article Snippet: Indirect immunofluorescence assay Indirect immunofluorescence assay (IFA) was done using commercial IFA slides with PUUV (strain Hällnäs B1 83–223L)-, HTNV (strain 76–118)- and SEOV (strain R22)-infected Vero E6 cells (Progen Biotechnik, Heidelberg, Germany) and PUUV (strain Sotkamo)-, DOBV (strain Slovenia)-, Saaremaa virus-, SEOV-, HTNV- and SNV-infected Vero E6 cells (EuroImmun, Lübeck, Germany) as recommended by the manufacturers.

    Techniques: Recombinant

    Reactivity of N-protein-specific mAbs with hantavirus-infected Vero E6 cells in commercial and in-house indirect IFA

    Journal: Archives of virology

    Article Title: Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples

    doi: 10.1007/s00705-010-0879-6

    Figure Lengend Snippet: Reactivity of N-protein-specific mAbs with hantavirus-infected Vero E6 cells in commercial and in-house indirect IFA

    Article Snippet: Indirect immunofluorescence assay Indirect immunofluorescence assay (IFA) was done using commercial IFA slides with PUUV (strain Hällnäs B1 83–223L)-, HTNV (strain 76–118)- and SEOV (strain R22)-infected Vero E6 cells (Progen Biotechnik, Heidelberg, Germany) and PUUV (strain Sotkamo)-, DOBV (strain Slovenia)-, Saaremaa virus-, SEOV-, HTNV- and SNV-infected Vero E6 cells (EuroImmun, Lübeck, Germany) as recommended by the manufacturers.

    Techniques: Virus

    Multiple amino acid (aa) sequence alignment of the amino-terminal region (aa 1–120) of the N proteins of Puumala virus strains Kazan (PUUV-Kaz, GenBank accession No. Z84204), P-360 (PUUVP360, L11347), Sotkamo (PUUV-Sot, X61035), Vranica/Hällnäs (PUUV-Vra, U14137), Tula virus, strain Moravia (TULV, Z69991), Prospect Hill virus (PHV, M34011), Bayou virus (BAYV, L36929), Muleshoe virus (MULEV, U54575), Sin Nombre virus (SNV, NC_005216), Andes virus (ANDV, AF004660), Rio Mamore virus (RMV, U52136), Hantaan virus, strain Fojnica (HTNV, M14626), Seoul virus (SEOV, AY273791) and Dobrava-Belgrade virus, strain Slovenia (DOBV-Slo, L41916). The alignment was generated using ClustalW software. “*” indicates identical aa residues in all sequences in the alignment, “:” indicates conserved substitutions, and “.” indicates semi-conserved substitutions in the alignment

    Journal: Archives of virology

    Article Title: Characterization of monoclonal antibodies against hantavirus nucleocapsid protein and their use for immunohistochemistry on rodent and human samples

    doi: 10.1007/s00705-010-0879-6

    Figure Lengend Snippet: Multiple amino acid (aa) sequence alignment of the amino-terminal region (aa 1–120) of the N proteins of Puumala virus strains Kazan (PUUV-Kaz, GenBank accession No. Z84204), P-360 (PUUVP360, L11347), Sotkamo (PUUV-Sot, X61035), Vranica/Hällnäs (PUUV-Vra, U14137), Tula virus, strain Moravia (TULV, Z69991), Prospect Hill virus (PHV, M34011), Bayou virus (BAYV, L36929), Muleshoe virus (MULEV, U54575), Sin Nombre virus (SNV, NC_005216), Andes virus (ANDV, AF004660), Rio Mamore virus (RMV, U52136), Hantaan virus, strain Fojnica (HTNV, M14626), Seoul virus (SEOV, AY273791) and Dobrava-Belgrade virus, strain Slovenia (DOBV-Slo, L41916). The alignment was generated using ClustalW software. “*” indicates identical aa residues in all sequences in the alignment, “:” indicates conserved substitutions, and “.” indicates semi-conserved substitutions in the alignment

    Article Snippet: Indirect immunofluorescence assay Indirect immunofluorescence assay (IFA) was done using commercial IFA slides with PUUV (strain Hällnäs B1 83–223L)-, HTNV (strain 76–118)- and SEOV (strain R22)-infected Vero E6 cells (Progen Biotechnik, Heidelberg, Germany) and PUUV (strain Sotkamo)-, DOBV (strain Slovenia)-, Saaremaa virus-, SEOV-, HTNV- and SNV-infected Vero E6 cells (EuroImmun, Lübeck, Germany) as recommended by the manufacturers.

    Techniques: Sequencing, Virus, Generated, Software

    Journal: bioRxiv

    Article Title: Bank vole immunoheterogeneity may limit Nephropatia Epidemica emergence in a French non-endemic region

    doi: 10.1101/130252

    Figure Lengend Snippet:

    Article Snippet: 1.7x10 3 f.f.u of PUUV Sotkamo strain 1:10 diluted in DMEM (ThermoFisher Scientific) were injected by subcutaneous route in each of the 19 bank voles.

    Techniques:

    Variation of the optical density in ELISA test ( OD 450nm ) over time for the 14 bank voles that seroconverted during the experimental infections. Barplots and error bars represent the mean OD 450nm value ± SD for all the individuals in each region. The hatched line represents the threshold above which the bank voles were considered to be PUUV seropositive.

    Journal: bioRxiv

    Article Title: Bank vole immunoheterogeneity may limit Nephropatia Epidemica emergence in a French non-endemic region

    doi: 10.1101/130252

    Figure Lengend Snippet: Variation of the optical density in ELISA test ( OD 450nm ) over time for the 14 bank voles that seroconverted during the experimental infections. Barplots and error bars represent the mean OD 450nm value ± SD for all the individuals in each region. The hatched line represents the threshold above which the bank voles were considered to be PUUV seropositive.

    Article Snippet: 1.7x10 3 f.f.u of PUUV Sotkamo strain 1:10 diluted in DMEM (ThermoFisher Scientific) were injected by subcutaneous route in each of the 19 bank voles.

    Techniques: Enzyme-linked Immunosorbent Assay

    Cells grown on coverslips were infected at a MOI of 0.1, fixed and stained (in green) for PUUV Kazan-E6 at 5 days post infection (dpi) (A), or for PUUV Umeå at 11 dpi (B). Nuclei were visualized with DAPI staining (blue). Cultivation media from VEFs infected with PUUV Kazan-E6 (C) or PUUV Umeå (D) were collected at the indicated time points post infection and the virus titre, determined as focus forming units (FFU)/ml, were assayed. Graphs represent the means from one of two or more different experiments.

    Journal: PLoS ONE

    Article Title: A Model System for In Vitro Studies of Bank Vole Borne Viruses

    doi: 10.1371/journal.pone.0028992

    Figure Lengend Snippet: Cells grown on coverslips were infected at a MOI of 0.1, fixed and stained (in green) for PUUV Kazan-E6 at 5 days post infection (dpi) (A), or for PUUV Umeå at 11 dpi (B). Nuclei were visualized with DAPI staining (blue). Cultivation media from VEFs infected with PUUV Kazan-E6 (C) or PUUV Umeå (D) were collected at the indicated time points post infection and the virus titre, determined as focus forming units (FFU)/ml, were assayed. Graphs represent the means from one of two or more different experiments.

    Article Snippet: Wild type PUUV strain Kazan (PUUV-wt) , Vero E6 cell line-adapted PUUV strain Kazan (PUUV Kazan-E6) and Umeå/305/human/95 (PUUV Umeå) , TBEV strain 93–783 , CPXV strain ATCC VR 302, LV strain 145SLG , and green fluorescent protein-expressing Newcastle disease virus (NDV-GFP) were used in the present study.

    Techniques: Infection, Staining, Virus

    Cells grown on coverslips were infected with indicated viruses at a MOI of 0.1, fixed and stained (in green) for CPXV at 17 hours post infection (hpi) (A), LV at 8 hpi (B), and for TBEV at 24 hpi (C). Nuclei were visualized with DAPI staining (blue). To measure virus production, VEFs were infected with CPXV at a MOI of 0.1 (D), LV at a MOI of 60 (E) or TBEV at a MOI of 100 (F) and cultivation media was collected at the indicated time points post infection. Virus progeny production (virus/ml cell culture medium) was determined by titration on Vero E6. Error bars represent standard deviations of the means from one experiment.

    Journal: PLoS ONE

    Article Title: A Model System for In Vitro Studies of Bank Vole Borne Viruses

    doi: 10.1371/journal.pone.0028992

    Figure Lengend Snippet: Cells grown on coverslips were infected with indicated viruses at a MOI of 0.1, fixed and stained (in green) for CPXV at 17 hours post infection (hpi) (A), LV at 8 hpi (B), and for TBEV at 24 hpi (C). Nuclei were visualized with DAPI staining (blue). To measure virus production, VEFs were infected with CPXV at a MOI of 0.1 (D), LV at a MOI of 60 (E) or TBEV at a MOI of 100 (F) and cultivation media was collected at the indicated time points post infection. Virus progeny production (virus/ml cell culture medium) was determined by titration on Vero E6. Error bars represent standard deviations of the means from one experiment.

    Article Snippet: Wild type PUUV strain Kazan (PUUV-wt) , Vero E6 cell line-adapted PUUV strain Kazan (PUUV Kazan-E6) and Umeå/305/human/95 (PUUV Umeå) , TBEV strain 93–783 , CPXV strain ATCC VR 302, LV strain 145SLG , and green fluorescent protein-expressing Newcastle disease virus (NDV-GFP) were used in the present study.

    Techniques: Infection, Staining, Virus, Cell Culture, Titration

    TABLE 1.

    Journal: Journal of Virology

    Article Title: Interactions and Oligomerization of Hantavirus Glycoproteins ▿

    doi: 10.1128/JVI.00481-09

    Figure Lengend Snippet: TABLE 1.

    Article Snippet: The Puumala virus (PUUV) Sotkamo strain and Tula virus (TULV) Moravia strain 5302 were cultivated in Vero E6 green monkey kidney epithelial cells (ATCC 94 CRL-1586).

    Techniques: